Journal: Journal of Clinical and Translational Hepatology

Article Title: CEACAM-1 Induced CSF3-receptor Downregulation in Bone Marrow Associated With Refractory Neutropenia in Advanced Cirrhosis

doi: 10.14218/JCTH.2021.00331

Figure Lengend Snippet: (A) Granulocyte colony-stimulating factor (G-CSF) levels in the bone marrow plasma and peripheral blood plasma of controls and (mean±SD). (B) Colony-stimulating factor receptor (CSF3R) levels in the bone marrow plasma and peripheral blood plasma of control and cirrhosis cases by CTP-A, -B, and -C (mean±SD). (C) Quantitative RT-PCR of colony-stimulating factor receptor 3 ( CSF3R ) mRNA in bone marrow biopsies (mean±SD), n =5 each. (D) Representative images of Immunohistochemistry (IHC) expression of CSF3R in bone marrow biopsies of control and Child-Turcott-Pugh (CTP)-A, B-, C- classes of cirrhosis, all cases. (E) Comparison of baseline colony-stimulating factor receptor (CSFR) levels in bone marrow plasma and peripheral blood plasma of cirrhotic patients with naturally acquired infection during follow-up, and (F) cirrhotic patients who had leukopenia and with normal total leukocyte count, all cases. ** p <0.001, * p =0.001 to <0.05; ns, not-significant.

Article Snippet: ELSA of human G-CSF (E-EL-H0079) and CSF3R (E-EL-HO799, both from Elabscience, Hubei, China, and CEACAM-1 (CSB-EL005157HU, Cusabio, Hubei, China) were performed on BM and peripheral blood (PB) plasma from cirrhosis patients and control participants.

Techniques: Quantitative RT-PCR, Immunohistochemistry, Expressing, Infection

Journal: Journal of Clinical and Translational Hepatology

Article Title: CEACAM-1 Induced CSF3-receptor Downregulation in Bone Marrow Associated With Refractory Neutropenia in Advanced Cirrhosis

doi: 10.14218/JCTH.2021.00331

Figure Lengend Snippet: (A) Area under the curve of circulatory colony-stimulating factor receptor (CSF3R) level in peripheral blood for neutropenia and (B) infection. (C) Baseline Colony-stimulating factor receptor (CSF3R) in peripheral blood plasma from the original cohort ( n =15) and (D) Validation cohort ( n =22) of cirrhosis patients who responded to granulocyte- colony stimulating factor (GCSF) treatment for neutropenia vs. nonresponders {median, interquartile range (IQR) }. ** p <0.001, * p =0.001 to <0.05; ns, not significant.

Article Snippet: ELSA of human G-CSF (E-EL-H0079) and CSF3R (E-EL-HO799, both from Elabscience, Hubei, China, and CEACAM-1 (CSB-EL005157HU, Cusabio, Hubei, China) were performed on BM and peripheral blood (PB) plasma from cirrhosis patients and control participants.

Techniques: Infection

Journal: Journal of Clinical and Translational Hepatology

Article Title: CEACAM-1 Induced CSF3-receptor Downregulation in Bone Marrow Associated With Refractory Neutropenia in Advanced Cirrhosis

doi: 10.14218/JCTH.2021.00331

Figure Lengend Snippet: (A) Representative images of flow cytometry of bone marrow aspirates with acquired CD34+ cells (%) and CSF3R+CD34+ cells (%) in control and cirrhosis Child-Turcott-Pugh (CTP) classes A,B,C), n =25 each. (B) Distribution of CD34+ cells % in control and cirrhosis (CTP classes A, B, C), median, interquartile range (IQR), n =25 each. (C) Distribution of CSF3R+CD34+ (%) of total bone marrow C34+ cells, median (interquartile range), n =25 each. (D) Distribution of granulocyte monocyte-colony forming unit (GM-CFU) and burst forming unit-erythroid (BFU-E) across the study groups, median (interquartile range), n =10 each. ** p <0.001, * p =0.001 to <0.05; ns, not significant.

Article Snippet: ELSA of human G-CSF (E-EL-H0079) and CSF3R (E-EL-HO799, both from Elabscience, Hubei, China, and CEACAM-1 (CSB-EL005157HU, Cusabio, Hubei, China) were performed on BM and peripheral blood (PB) plasma from cirrhosis patients and control participants.

Techniques: Flow Cytometry

Journal: Journal of Clinical and Translational Hepatology

Article Title: CEACAM-1 Induced CSF3-receptor Downregulation in Bone Marrow Associated With Refractory Neutropenia in Advanced Cirrhosis

doi: 10.14218/JCTH.2021.00331

Figure Lengend Snippet: (A) mRNA expression of, carcinoembryonic antigen cell adhesion molecule-1 ( CEACAM-1) gene in the bone marrow biopsies of control and cirrhosis classes (mean±SD), n =5. (B) Representative images and graphical representation of CEACAM-1+ bone marrow cells in control and cirrhosis classes (20×). (C) Graphical representation of CEACAM-1 in bone marrow plasma (mean±SD). (D) CEACAM-1 levels in the peripheral blood plasma of control and cirrhosis classes (mean±SD), and (E) representative images of flow cytometry of bone marrow mononuclear cultured cells display the difference in CD34+CSF3R+ cells without CEACAM-1 and with CEACAM-1 treatment for 24 h, n =5. ** p <0.001, * p =0.001 to <0.05; ns, not significant.

Article Snippet: ELSA of human G-CSF (E-EL-H0079) and CSF3R (E-EL-HO799, both from Elabscience, Hubei, China, and CEACAM-1 (CSB-EL005157HU, Cusabio, Hubei, China) were performed on BM and peripheral blood (PB) plasma from cirrhosis patients and control participants.

Techniques: Expressing, Flow Cytometry, Cell Culture

Journal: Journal of Clinical and Translational Hepatology

Article Title: CEACAM-1 Induced CSF3-receptor Downregulation in Bone Marrow Associated With Refractory Neutropenia in Advanced Cirrhosis

doi: 10.14218/JCTH.2021.00331

Figure Lengend Snippet: (A) C-X-C chemokine receptor type 4 (CXCR4+) cells in cytospin smears of sorted CD34 cells from bone marrow in the control and cirrhosis classes (Giemsa, 20×), and (B) Graph of distribution, median (interquartile range), n =10 each. (C) Distribution of CSF3R−TLR3+ CD34 cells, and (D) CSF3R+TLR3− CD34 cells in control and cirrhotic classes, median (interquartile range), n =25 each. (E) Representative images of ubiquitin-positive cells/20 fields in the bone marrow of study groups (20×) and (F) graphical representation of the cells across the study groups, median (interquartile range), all cases. ** p <0.001, * p =0.001 to <0.05; ns, not significant.

Article Snippet: ELSA of human G-CSF (E-EL-H0079) and CSF3R (E-EL-HO799, both from Elabscience, Hubei, China, and CEACAM-1 (CSB-EL005157HU, Cusabio, Hubei, China) were performed on BM and peripheral blood (PB) plasma from cirrhosis patients and control participants.

Techniques:

Journal: Scientific Reports

Article Title: Proteomic analysis of serum extracellular vesicles from biliary tract infection patients to identify novel biomarkers

doi: 10.1038/s41598-024-56036-y

Figure Lengend Snippet: The top 15 upregulated proteins of differential expressed proteins.

Article Snippet: Protein levels were determined using ELISA kits according to the manufacturer’s instructions: Human CEACAM1 ELISA Kit (EK1361, BOSTER, China) and Human Protein Crumbs Homolog 3 (CRB3) ELISA Kit (abx386665, abbexa, UK).

Techniques:

Journal: Scientific Reports

Article Title: Proteomic analysis of serum extracellular vesicles from biliary tract infection patients to identify novel biomarkers

doi: 10.1038/s41598-024-56036-y

Figure Lengend Snippet: Validation of CEACAM1 and CRB3 expression in serum EVs. ( A ) CEACAM1 and CRB3 were detectable by Western blot (WB) analysis (N total = 8). ( B ) Densitometric quantification of CEACAM1. ( C ) Densitometric quantification of CRB3. ( D ) CRB3 was detectable by ELISA analysis. ( E ) CEACAM1 was detectable by ELISA analysis. * p < 0.05.

Article Snippet: Protein levels were determined using ELISA kits according to the manufacturer’s instructions: Human CEACAM1 ELISA Kit (EK1361, BOSTER, China) and Human Protein Crumbs Homolog 3 (CRB3) ELISA Kit (abx386665, abbexa, UK).

Techniques: Biomarker Discovery, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay